Hi all
I need to digest the yeast genome for separation on a gel before
probing for my inserted gene of interest. I know which enzymes I do not
want to use (anything that cuts within my gene is obviuosly not on) how
do i decide which enzyme to use. Any more scientific approach than
picking a few 6bp cutters from the endless list and see what the outcome
is?
Help would be appreciated thanx.
Joseline
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