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yeast mRNA isolation

anna ballew aballew at stanford.edu
Wed Dec 1 18:18:38 EST 2004

Hi - I am trying to set up a laboratory exercise for a course I am teaching 
to sophomores.  We want to transcription microarrays and last year we use 
typical methods for RNA isolation including bead beating and hot phenol.  I 
recently saw that a company Epicentre makes a kit (MasterPure RNA isolation 
kit) that supposedly doesn't use either beads or phenol.  I'm assuming that 
there is some type of enzymatic method that breaks open the yeast, but I 
can't tell from their protocol.  And I think you spin the lysate over some 
column that binds to the RNA.  I'm wondering if anyone has ever used this 
kit or one like it.  It's attractive in that if it works, I could avoid 
having 20 sophomores wrestling tubes of hot phenol.  But it would be a total 
waste of my time and money if the RNA you get from using this kit is crappy. 
Any suggestions would be welcomed.

Anna Ballew
Stanford University 

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