I have successfully been using the GAL4-based yeast two-hybrid system.
But I also want to try the lexA base interaction trap for a particular
experiment. However, Clontech seems to have discontinued this one and
I am not aware of any other commercial source selling the "classical"
components. I am aware that Invitrogen has got one but this is somehow
a further develompent of the original system. Does anyone have the
"old" system and would be willing to share some of the stuff?
What I would like to have is:
Yeast Strain: EGY48
lacZ reporter plasmid: pSH18-34
plasmids: pB42AD; pGilda, pJG-45-5
Any help is appreciated.
Thanks in advance.
boernke at ipk-gatersleben.de
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