I use the tet-off system, and I get very good repression on dox plates.
I don't prepare the plates with the dox, but add it afterwards (40=B5l of 1=
mg/ml,
and spread it all over the plate).
Gal
Christine Federovitch wrote:
> I am interested in conducting a conditional screen using the
> TET-inducible system. My doxycycline plates do not induce expression
> of my protein, however my protein induces normally in liquid culture
> with doxycycline. Has anyone successfully made antibiotic plates that
> induce the TET system? Or are there any suggestions?
>> I appreciate your help.
> Christine
> --
> Christine Federovitch
> Division of Biology
> University of California San Diego
> 2111 Pacific Hall, 0347
> 9500 Gilman Dr.
> La Jolla, CA 92093-0347
>> phone (858) 822-0512
> fax (858) 534-0555
> e-mail cfederov at biomail.ucsd.edu>> ---
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