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tet-induction on plates

Christine Federovitch cfederov at biomail.ucsd.edu
Tue Mar 11 15:11:24 EST 2003


I am interested in conducting a conditional screen using the
TET-inducible system. My doxycycline plates do not induce expression
of my protein, however my protein induces normally in liquid culture
with doxycycline. Has anyone successfully made antibiotic plates that
induce the TET system? Or are there any suggestions?

I appreciate your help.
Christine
-- 
Christine Federovitch
Division of Biology
University of California San Diego
2111 Pacific Hall, 0347
9500 Gilman Dr.
La Jolla, CA 92093-0347

phone (858) 822-0512
fax   (858) 534-0555
e-mail cfederov at biomail.ucsd.edu

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