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James Bond bc_1400 at hotmail.com
Thu Jun 26 07:14:27 EST 2003

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In article <b83m6h$avk$1 at mercury.hgmp.mrc.ac.uk>, Claudette C.
<claudettecoert at yahoo.com> wrote:

> Have been trying to use 5FOA to dedelect for URA+ to no avail. I've
> tried adding proline to the media in order to make the cells
> hypersensitive to 5FOA and have also tried replica plating to new 5FOA
> plates after initial growth.
> The problem is that after each try, instead of only a few cells
> growing, I find a lawn of cells. How many cfu/ml should I be spreading
> in the first place, and also is there anything I might be missing?
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