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Claudette C. claudettecoert at yahoo.com
Tue Apr 22 05:52:58 EST 2003

Have been trying to use 5FOA to dedelect for URA+ to no avail. I've
tried adding proline to the media in order to make the cells
hypersensitive to 5FOA and have also tried replica plating to new 5FOA
plates after initial growth.

The problem is that after each try, instead of only a few cells
growing, I find a lawn of cells. How many cfu/ml should I be spreading
in the first place, and also is there anything I might be missing?

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