Hi everybody !
When I tried to transform the two hybrid strain Y187 with a pRS411 construct
(MET15) I could not get any transformands. In the Matchmaker user guide the
Clontech people mention only met- for this strain, so I assumed it would be
met15 and used pRS411. After one hour of net-searching I found that it might
be met13 indeed (Mol Cells 2001 Jun 30;11(3):275-80, by Lee MY and Kim J).
Has anybody practical experience ? I wonder if could get a plasmid with
MET13 somewhere. My problem is simple: METxx is the only marker left in my
system.
Thank you for your help !
Eddie
--
GMX - Die Kommunikationsplattform im Internet.
http://www.gmx.net
---
______________O_________oO_____________oO______o_______oO___________________
YEAST bionet newsgroup see: http://www.bio.net/hypermail/YEAST/
YEAST e-mail: messages sent to yeast at net.bio.net
subscribe: e-mail biosci-server at net.bio.net with: subscribe yeast
unsubscribe: e-mail biosci-server at net.bio.net with: unsubscribe yeast
YEAST on the WWW: http://genome-www.stanford.edu/Saccharomyces/VL-yeast.html
problems with the YEAST newsgroup? E-mail the moderator: francis at cmmt.ubc.ca
