You may be able to get away with plating on Rafinose. Once the colonies
have formed, lift onto raf/gal (2%) plates for 4hrs to induce cDNA
expression. Why do you think the cDNA's will be toxic?
Martin Hirst Ph.D
Interomex Biopharmaceuticals Inc.
3590 West 41st Ave.
direct line:(604) 267-8005
home: (604) 943-5856
fax: (604) 267-6411
email: hirst at interomex.comhttp://www.interomex.com
> I searched the archives, but didn't find the answer.
>> I have a library in pYES2, for induction with galactose. It would be ideal
> to turn on expression of the cDNA on filter lifts, which would then be used
> to assay for the desired phenotype. I am aware of this being done in liquid
> culture, but does anyone have or know of a protocol to do this on filters?
>> Our assumption is that the protein expressed will be toxic for yeast,
> therefore I don't believe that we could recover the colonies after induction
> by plating on Gal media.
>> If this is a trivial question, please forgive my ignorance.
>> Mark T. Worthington
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