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YNB fluorescence

Kurt Thorn Kthorn at CGR.Harvard.edu
Fri May 17 14:46:56 EST 2002

Hi all -

I am trying to use CFP as a fusion tag for fluorescence measurements in
yeast.  However, I find that YNB is highly fluorescent when excited at 450
nm and completely swamps my CFP signal.  has anyone seen this problem
before?  Does anyone know of a good source for low fluorescence YNB or a
good replacement for it?


Kurt Thorn
Bauer Center for Genomics Research, Room 206
Harvard University
7 Divinity Ave.
Cambridge MA 02138
(617) 384-9456
kthorn at cgr.harvard.edu


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