> > Does anyone know where I can get stains to complement respiratory
> > deficiency due to mutations in mitochondrial DNA? Thanks.
> > Paul Kirchman
> > Florida Atlantic University
> > pkirchma at fau.edu>> Are you asking for a wild type strain? Could you complement with a ARS-CEN
> mitochondrial DNA library?
>>> Martin Hirst
No, I don't think that you can complement mitochondrial DNA mutations
with cen-ars plasmids, which would be in the nucleus. I am looking
for strains that contain known mutations of mitochondrial DNA (ie.
cox1, atp8) that can be mated with my strain to complement respiratory
defeciency. ATCC has a few (cox2, cox3, atp6, cob), but I would also
need atp9, cox1, atp8, var1, and possibly others.
Florida Atlantic University
pkirchma at fau.edu
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