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low expression promoters in yeast

Martin Hirst hirst4 at interomex.com
Tue May 7 06:24:34 EST 2002


On 5/7/02 1:05 AM, "medicherla bala subrahmanyam"
<subrahmanyam at po.uni-stuttgart.de> wrote:

> hello
> iam lokking for the promoters whose expression will be very low for the
> expression of my protein in yeast , for the screening compulsions i have
> to use prs316 plasmid of yeast only i tried to express my protein under
> its own promoter but its expression is more than what i want , so iam
> looking for the promoters which shows low level of expression through
> out the cell cycle and there growth
> 
> thank you
> bala s medicherla
> mb_subbu at yahoo.com
> 
Good Day Bala,

The natural GAL4 promoter is extremely weak especially if you are growing in
SD media.  See Griggs and Johnston MCB 13 (8) 4999-5009.

Regards

Martin





-------------------------------------------
 
Martin Hirst Ph.D


Interomex Biopharmaceuticals Inc.
3590 West 41st Ave.
Vancouver, BC
Canada
V6N 3E6



 



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