On 5/7/02 1:05 AM, "medicherla bala subrahmanyam"
<subrahmanyam at po.uni-stuttgart.de> wrote:
> iam lokking for the promoters whose expression will be very low for the
> expression of my protein in yeast , for the screening compulsions i have
> to use prs316 plasmid of yeast only i tried to express my protein under
> its own promoter but its expression is more than what i want , so iam
> looking for the promoters which shows low level of expression through
> out the cell cycle and there growth
>> thank you
> bala s medicherla
>mb_subbu at yahoo.com>Good Day Bala,
The natural GAL4 promoter is extremely weak especially if you are growing in
SD media. See Griggs and Johnston MCB 13 (8) 4999-5009.
Martin Hirst Ph.D
Interomex Biopharmaceuticals Inc.
3590 West 41st Ave.
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