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Yeast Antibiotic Resistant Genes

Jianhua Yu yu19 at purdue.edu
Mon Jun 10 17:14:59 EST 2002


Hello, yeast colleagues,

I use G418 as antibiotic in yeast Saccharomyces cerevisiae. But it seems
that the non-resistant strains can also grow on G418 plates (maybe
because our G418 is too old). I think many of you use MxKan4 resistant
gene as a selectable marker in yeast. Does anybody can give me some
suggestions about this selectable marker? For example, usually where to
buy G418 (Geneticin), Sigma? How to make plates (Sc or SD) to select
transformants? Is it a good selectable marker?

Furthermore, does anybody know some other antibiotic selectable markers
(positive or negative) which can be used in yeast?

I am not very familiar with yeast. Sorry about this simple question and
appreciate your time.

Thanks for your help!

Jianhua

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