On 4 Jun 2002, Trent Bosma wrote:
> Yeast two hybrid (ProQuest system using ResGen Hela cDNA library)
>> 1. The majority of my putative interactors contain more than 1 cDNA. Has
> anyone experienced similar results and found a cure apart from changing
> my bait?
When you transform yeast with a library, it is normal for some of the
transformants to carry more than one plasmid. You need to clone the
plasmids into E.coli, then re-transform the isolated plasmids
independently to determine which one is giving you the interaction.
Alex
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