Yeast two hybrid (ProQuest system using ResGen Hela cDNA library)
1. The majority of my putative interactors contain more than 1 cDNA. Has
anyone experienced similar results and found a cure apart from changing
my bait?
2. Has anyone successfully extracted the plasmids out of the controls
provided with the kit i.e. what is the antibiotic marker of pPC97?. I was
expecting it to be kanamycin as with pPC86 but it doesn’t appear to be so.
Trent J. Bosma PhD.
LCI/NIAID/NIH
Bldg. 10/11N222
301 496 7680
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