IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

Yeast lysis with Robot

James Delproposto delpropo at msu.edu
Wed Jul 31 10:02:03 EST 2002

I have been trying to develop a protocol to lyse yeast cells using a
robot for plasmid preps.  The only functionality that the robot has is
pipeting, shaking, and vacum filtration which takes the places of
centrifuging.  I doesnt have the ability to vortex, so glass beads are
out of the question.  It cannot incubate at higher than room
temperature so that rules out zymolase and a couple other methods.  If
anyone has a method that can lyse s. cer with only reagents and
shaking, please let me know.

YEAST bionet newsgroup see: http://www.bio.net/hypermail/YEAST/
YEAST e-mail: messages sent to yeast at net.bio.net
subscribe: e-mail biosci-server at net.bio.net with: subscribe yeast
unsubscribe: e-mail biosci-server at net.bio.net with: unsubscribe yeast
YEAST on the WWW: http://genome-www.stanford.edu/Saccharomyces/VL-yeast.html
problems with the YEAST newsgroup? E-mail the moderator: francis at cmmt.ubc.ca

More information about the Yeast mailing list

Send comments to us at biosci-help [At] net.bio.net