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Homologous Recombo Library Construction

Michael Hunter mhunter0 at jomail.jo.ecu.edu.au
Tue Jul 23 01:01:59 EST 2002

Recently I performed a Y2H screen using Clontechs MMIII Library
construction and screening kit. In this system a library of your choice
is constructed and propagated in a yeast strain by homologous
recombination. I would like to be able to shuttle this library to a
bacterial strain but fear that the low copy number of library plasmids
derived from a "mass" yeast plasmid prep and subsequent difficulties in
successfully transforming competent bacteria, would result in a less
than satisfactory library representation. To overcome this would it be
possible to transform a suitable bacteria strain with the linearised
vector and dscDNA library inserts? Does anyone know of a bacterial
strain that can perform homologous recombination? Or is this just a
process that occurs in yeast?

Michael Hunter
Centre for Human Genetics
Edith Cowan University
100 Joondalup Drive
Ph. 9400 5716
Mob. 0419 367 091
Fax. 9400 5871

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