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Homologous Recombo Library Construction

Michael Hunter mhunter0 at jomail.jo.ecu.edu.au
Tue Jul 23 01:01:59 EST 2002


Hi,
Recently I performed a Y2H screen using Clontechs MMIII Library
construction and screening kit. In this system a library of your choice
is constructed and propagated in a yeast strain by homologous
recombination. I would like to be able to shuttle this library to a
bacterial strain but fear that the low copy number of library plasmids
derived from a "mass" yeast plasmid prep and subsequent difficulties in
successfully transforming competent bacteria, would result in a less
than satisfactory library representation. To overcome this would it be
possible to transform a suitable bacteria strain with the linearised
vector and dscDNA library inserts? Does anyone know of a bacterial
strain that can perform homologous recombination? Or is this just a
process that occurs in yeast?
Thankyou

Michael Hunter
Centre for Human Genetics
Edith Cowan University
100 Joondalup Drive
Joondalup
WESTERN AUSTRALIA
6027
Ph. 9400 5716
Mob. 0419 367 091
Fax. 9400 5871
http://www.chg.ecu.edu.au/people/students/michael_hunter.html
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