IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP


Peter Kosa pkosa at kosatka.fns.uniba.sk
Sat Jul 20 09:58:42 EST 2002

Hi everybody,
I want to use blasticidine cassette for PCR gene disruption in yeast. I would like to known how to plate transformants. First non-selective medium (how long?) and then selective (containing blasticidin) or direct plating transformatns on selective medium with antibiotic?

Peter Kosa
Department of Biochemistry
Faculty of Natural Sciences
Comenius University
Mlynska dolina CH-1
84215 Bratislava
E-mail: pkosa at st.fns.uniba.sk

YEAST bionet newsgroup see: http://www.bio.net/hypermail/YEAST/
YEAST e-mail: messages sent to yeast at net.bio.net
subscribe: e-mail biosci-server at net.bio.net with: subscribe yeast
unsubscribe: e-mail biosci-server at net.bio.net with: unsubscribe yeast
YEAST on the WWW: http://genome-www.stanford.edu/Saccharomyces/VL-yeast.html
problems with the YEAST newsgroup? E-mail the moderator: francis at cmmt.ubc.ca

More information about the Yeast mailing list

Send comments to us at biosci-help [At] net.bio.net