Hi everybody,
I want to use blasticidine cassette for PCR gene disruption in yeast. I would like to known how to plate transformants. First non-selective medium (how long?) and then selective (containing blasticidin) or direct plating transformatns on selective medium with antibiotic?
Thanks...
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Peter Kosa
Department of Biochemistry
Faculty of Natural Sciences
Comenius University
Mlynska dolina CH-1
84215 Bratislava
Slovakia
http://www.fns.uniba.sk/~kbi/kovlab/
E-mail: pkosa at st.fns.uniba.sk
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