How long does mRNA survive in dead cells?
On average, how long does mRNA survive in S. cerevisiae after
cell death? We're contemplating a microarray experiment for which the
control is likely to have a significant fraction of dead cells. If dead
cells have little to no intact mRNA, this shouldn't be a serious
problem. On the other hand, it they do, it will introduce complications.
Does anyone know of studies that have tried to detect total mRNA or
specific species post-mortem or that have looked at mRNA survival as a
function of cause of death?
Thanks for your help,
YEAST bionet newsgroup see: http://www.bio.net/hypermail/YEAST/
YEAST e-mail: messages sent to yeast at net.bio.net
subscribe: e-mail biosci-server at net.bio.net with: subscribe yeast
unsubscribe: e-mail biosci-server at net.bio.net with: unsubscribe yeast
YEAST on the WWW: http://genome-www.stanford.edu/Saccharomyces/VL-yeast.html
problems with the YEAST newsgroup? E-mail the moderator: francis at cmmt.ubc.ca