IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

Transposon mutagenesis

akabir anaul_kabir at urmc.rochester.edu
Sun Jan 27 19:21:21 EST 2002

Dear Everybody,

For transposon mediated gene disruption, we generally make the cassette in 
vitro and then linearize it and transform into yeast which land in the 
yeast genome and inserts by homologous recombination. To avoid that 
procedure, I am thinking of using Tn7 or Tn3 transposon to transform into 
yeast directly and to disrupt a gene. Does anybody have any idea about it?
    Please email me if you have some information.



YEAST bionet newsgroup see: http://www.bio.net/hypermail/YEAST/
YEAST e-mail: messages sent to yeast at net.bio.net
subscribe: e-mail biosci-server at net.bio.net with: subscribe yeast
unsubscribe: e-mail biosci-server at net.bio.net with: unsubscribe yeast
YEAST on the WWW: http://genome-www.stanford.edu/Saccharomyces/VL-yeast.html
problems with the YEAST newsgroup? E-mail the moderator: francis at cmmt.ubc.ca

More information about the Yeast mailing list

Send comments to us at biosci-help [At] net.bio.net