I'm looking for a high efficiency transformation method for use with the pombe mutant spc1/sty1.
I have tried electroporation, but am aware from previous experience that the transformation efficiency in this strain is around 10% against wild type.
I have also tried lithium acetate, but the 42 degree heat shock tends to kill spc1!!
Does anyone have any ideas or novel transformation protocols?
Chair of the Social and Cultural Committee
Cell Cycle/DNA Damage Research Groups
Radiation and Genome Stability Unit
YEAST bionet newsgroup see: http://www.bio.net/hypermail/YEAST/
YEAST e-mail: messages sent to yeast at net.bio.net
subscribe: e-mail biosci-server at net.bio.net with: subscribe yeast
unsubscribe: e-mail biosci-server at net.bio.net with: unsubscribe yeast
YEAST on the WWW: http://genome-www.stanford.edu/Saccharomyces/VL-yeast.html
problems with the YEAST newsgroup? E-mail the moderator: francis at cmmt.ubc.ca