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Yeast lysis

David O'Connor D.I.Oconnor at liverpool.ac.uk
Mon Feb 4 15:42:28 EST 2002

"Stephen Clark"  wrote
> I'm looking for a way to extract a protein from yeast without using
> glass beads.

There are a number of methods commonly used. Have you tried using a
sphaeroplasting enzyme such as Zymolyase or breaking the cells with
freeze-thaw in liquid nitrogen?

Best wishes,



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