IUBio

Bacteriomatch

bong yambao maria at res.agr.hokudai.ac.jp
Tue Nov 20 19:21:54 EST 2001


It was a failure in our part. After cotransformation of pBT (with insert-plant virus)
and non-recombinant pTRG, colonies grew on LBCTCK plates. We tried to alter
residues of our gene but it was still a failure.


>Hello, just wondering if anyone out there is using Stratagene's
>BacterioMatch two-hybrid system and what kind of results are you
>having.  My biggest question is if you are studying at eukaryotic
>protein as bait, does the protein folding in bacteria appear to be
>okay (e.g. protein still maintains activity with known
>controls/binding partners)?  Also, is there anything about the system
>that is not as good as yeast two-hybrid.  And do you recommend
>purchasing the system?
>
>Thanks.
>-Tom
>
>---





______________O_________oO_____________oO______o_______oO___________________
YEAST bionet newsgroup see: http://www.bio.net/hypermail/YEAST/
YEAST e-mail: messages sent to yeast at net.bio.net
subscribe: e-mail biosci-server at net.bio.net with: subscribe yeast
unsubscribe: e-mail biosci-server at net.bio.net with: unsubscribe yeast
YEAST on the WWW: http://genome-www.stanford.edu/Saccharomyces/VL-yeast.html
problems with the YEAST newsgroup? E-mail the moderator: francis at cmmt.ubc.ca




More information about the Yeast mailing list

Send comments to us at biosci-help [At] net.bio.net