It was a failure in our part. After cotransformation of pBT (with insert-plant virus)
and non-recombinant pTRG, colonies grew on LBCTCK plates. We tried to alter
residues of our gene but it was still a failure.
>Hello, just wondering if anyone out there is using Stratagene's
>BacterioMatch two-hybrid system and what kind of results are you
>having. My biggest question is if you are studying at eukaryotic
>protein as bait, does the protein folding in bacteria appear to be
>okay (e.g. protein still maintains activity with known
>controls/binding partners)? Also, is there anything about the system
>that is not as good as yeast two-hybrid. And do you recommend
>purchasing the system?
>>Thanks.
>-Tom
>>---
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