IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP


Alex Grant grant at zoology.ubc.ca
Thu May 24 12:50:25 EST 2001


I am trying to do a mutation rate experiment on my yeast by looking for
prototrophic colonies on antibiotic plates.  Unfortunately my yeast seems to
be completely resistant to Kanamycin.  I cannot use chloramphenicol,
erythromycin, or oligomycin as these attack the mitochondria (selecting for
petite mutants).  I also need an antibiotic which selects for a dominant
resistance gene so cycloheximide and 5'FOA are no good (I am comparing
different ploidies).  Can anybody suggest an antibiotic that meets these
requirements?  Would Zeocin work, or would resistant colonies be too rare?




YEAST bionet newsgroup see: http://www.bio.net/hypermail/YEAST/
YEAST e-mail: messages sent to yeast at net.bio.net
subscribe: e-mail biosci-server at net.bio.net with: subscribe yeast
unsubscribe: e-mail biosci-server at net.bio.net with: unsubscribe yeast
YEAST on the WWW: http://genome-www.stanford.edu/Saccharomyces/VL-yeast.html
problems with the YEAST newsgroup? E-mail the moderator: francis at cmmt.ubc.ca

More information about the Yeast mailing list

Send comments to us at biosci-help [At] net.bio.net