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Kozak sequences and yeast

Dr.Duncan Clark news at rutgers.edu
Fri Jul 13 08:37:00 EST 2001

Hi Folks,

I am having to get involved with yeast expression and am not sure if I
need both an rbs and a kozak sequence. We are planning to use the
Invitrogen vector pYC6/CT but need to ensure that our gene starts with
it's own ATG. The vector only has a GAL1 promoter so we presume we must
design in the kozak sequence. Do we therefore need another rbs? I'm so
used to expression in E.coli that I need to touch base on yeast.

Many thanks

The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
GeneSys Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382


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