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Re> S. pombe

SLF notmyaddress at hotmail.com
Tue Jul 10 14:23:02 EST 2001

> From: Amna Saeed (asaeed at research.neu.edu)
> Hi All
> I am new to the yeast world and am looking for some advice regarding growth
> / manipulation of S. pombe.

Some of your questions can be answered on our pombe technology web site or from links thereon
(see http://pingu.salk.edu/~forsburg/plasmids.html)

The inoculum you use can be calculated using standard microbiological methods but you will have to know the growth rate of your strain.  Generally your starter
should be quite dense for inoculation of your larger culture.

If you are selecting for a plasmid, you do want to go into EMM;  if the protein is toxic, you simply turn off the promoter.

Hope this helps,

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S L Forsburg, PhD  Associate Professor
Molecular Biology and Virology Lab
The Salk Institute, La Jolla CA

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