Biochemistry and Genetics of Eukaryotic RNA Expression
National Institutes of Health, USA
Two positions will be available to study the basic mechanisms of RNA
Investigations will involve a highly conserved pathway of tRNA
biogenesis that also
serves as a paradigm of eukaryotic gene expression. The functional
includes transcription, RNA processing, intranuclear trafficking of
complexes, and signal transduction. A multidisciplinary approach,
genetics, molecular biology and biochemistry, and a novel reporter
system in fission
yeast, will be used. Alu and tRNA-like retroposons are also
Background in molecular biology and biochemistry is essential.
1) Maraia, R. J. and Intine, R. V. (2001) Recognition of nascent RNA
by the human
La antigen: Conserved and diverged features of structure and function.
2) Intine RV, Sakulich AL, Koduru SB, Huang Y, Pierstorff E, Goodier
JL, Phan L,
Maraia RJ., (2000) Control of transfer RNA maturation by
phosphorylation of the
human La antigen on serine 366. Mol Cell, 6:339-348.
3) Huang Y, Hamada M, Maraia RJ. ( 2000) Isolation and cloning of four
of a fission yeast TFIIIC complex that includes an ortholog of the
protein TFIIIC-beta. J Biol Chem. Oct 6;275(40):31480-7.
4) Hamada M, Sakulich AL, Koduru SB, Maraia RJ. ( 2000) Transcription
termination by RNA polymerase III in fission yeast. A genetic and
tractable model system. J Biol Chem. Sep 15;275(37):29076-81.
5) Fan H, Sakulich AL, Goodier JL, Zhang X, Qin J, Maraia RJ. (1997)
Phosphorylation of the human La antigen on serine 366 can regulate
RNA polymerase III transcription complexes. Cell. Mar 7;88(5):707-15.
It is important that your application includes a statement detailing
your specific scientific interest in this position, a C.V., and 3
references. Send to: Richard J. Maraia, M.D., by E-mail:
maraiar at mail.nih.gov or post it to 6 Center Drive, MSC-2753,
Bethesda, MD, USA 20892-2753, or Fax to: 301 480-6863.
NIH is an equal opportunity employer
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