I am working on an essential multi-domain gene. Either of two exclusive
domains are sufficient to maintain vegetative growth. I have made a URA3
disruption (it's viable) and wish to replace MFG::URA with tagged alleles
in the chromosome. Has anyone had success with a one-step transformation
onto non-selective media followed by replica plating onto FOA? Because of
the multi-domain complementation, I don't want any partial duplications.
Relocation of the tagged genes to another locus is an alternative, I
admit, but would require more work.
YEAST bionet newsgroup see: http://www.bio.net/hypermail/YEAST/
YEAST e-mail: messages sent to yeast at net.bio.net
subscribe: e-mail biosci-server at net.bio.net with: subscribe yeast
unsubscribe: e-mail biosci-server at net.bio.net with: unsubscribe yeast
YEAST on the WWW: http://genome-www.stanford.edu/Saccharomyces/VL-yeast.html
problems with the YEAST newsgroup? E-mail the moderator: francis at cmmt.ubc.ca