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Deborah Field debbie.field at utoronto.ca
Wed Jan 17 13:05:31 EST 2001

Hi Folks,
	  I am working on an essential multi-domain gene. Either of two exclusive 
domains are sufficient to maintain vegetative growth.  I have made a URA3 
disruption (it's viable) and wish to replace MFG::URA with tagged alleles 
in the chromosome. Has anyone had success with a one-step transformation 
onto non-selective media followed by replica plating onto FOA? Because of 
the multi-domain complementation, I don't want any partial duplications. 
Relocation of the tagged genes to another locus is an alternative, I 
admit,  but would require more work.


Deborah Field


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