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HA tagging in S pombe

SLF notmyaddress at hotmail.com
Tue Feb 27 13:57:30 EST 2001

> I have been trying to HA tag a protein in S. pombe using the Bahler
> genomic tagging method but have had no sucess as yet (5 attempts and
> still going). To check the method of detection I am trying to find a
> vector to clone into to add the HA tag. Does anyone know of one and
> where I can get it from?
> Thanks,
> Trevor Pemberton
> (T.J.Pemberton at sussex.ac.uk)

Check out our HA tagging vectors at http://pingu.salk.edu/~forsburg/vectors.html#tag

Also, for an alternative strategy to the Bahler method, visit http://pingu.salk.edu/~forsburg/disruptions.html

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S L Forsburg, PhD  Associate Professor
Molecular Biology and Virology Lab
The Salk Institute, La Jolla CA

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