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pRS315 and XhoI?

anne rosenwald rosenwaa at georgetown.edu
Wed Feb 21 10:30:12 EST 2001


Hi everyone

the GenBank sequence of pRS315 predicts two XhoI sites, one in the
polylinker and one between LEU2 and f1.  However, the Sikorski and
Heiter paper says that  the XhoI site is good for cloning into the
polylinker.  Any thoughts?  (the direct experiment says that XhoI only
linearizes the plasmid).

Anne


Anne G. Rosenwald, Ph.D.
Assistant Professor of Biology
Georgetown University
406 Reiss Science Center
Box 571229
Washington, DC  20057-1229
phone (202) 687-5997
fax (202) 687-5662
email  rosenwaa at georgetown.edu


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