pRS315 and XhoI?
rosenwaa at georgetown.edu
Wed Feb 21 10:30:12 EST 2001
the GenBank sequence of pRS315 predicts two XhoI sites, one in the
polylinker and one between LEU2 and f1. However, the Sikorski and
Heiter paper says that the XhoI site is good for cloning into the
polylinker. Any thoughts? (the direct experiment says that XhoI only
linearizes the plasmid).
Anne G. Rosenwald, Ph.D.
Assistant Professor of Biology
406 Reiss Science Center
Washington, DC 20057-1229
phone (202) 687-5997
fax (202) 687-5662
email rosenwaa at georgetown.edu
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