IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

pRS315 and XhoI?

anne rosenwald rosenwaa at georgetown.edu
Wed Feb 21 10:30:12 EST 2001

Hi everyone

the GenBank sequence of pRS315 predicts two XhoI sites, one in the
polylinker and one between LEU2 and f1.  However, the Sikorski and
Heiter paper says that  the XhoI site is good for cloning into the
polylinker.  Any thoughts?  (the direct experiment says that XhoI only
linearizes the plasmid).


Anne G. Rosenwald, Ph.D.
Assistant Professor of Biology
Georgetown University
406 Reiss Science Center
Box 571229
Washington, DC  20057-1229
phone (202) 687-5997
fax (202) 687-5662
email  rosenwaa at georgetown.edu


YEAST bionet newsgroup see: http://www.bio.net/hypermail/YEAST/
YEAST e-mail: messages sent to yeast at net.bio.net
subscribe: e-mail biosci-server at net.bio.net with: subscribe yeast
unsubscribe: e-mail biosci-server at net.bio.net with: unsubscribe yeast
YEAST on the WWW: http://genome-www.stanford.edu/Saccharomyces/VL-yeast.html
problems with the YEAST newsgroup? E-mail the moderator: francis at cmmt.ubc.ca

More information about the Yeast mailing list

Send comments to us at biosci-help [At] net.bio.net