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Chromatin Immunoprecipitations

shwetal V.Mehta shwetal at mail.utexas.edu
Thu Dec 20 12:03:52 EST 2001


Hi,

I wanted to know if it is technically possible to immunoprecipitate 
intact chromosomes without fragmenting them? I have tried carrying 
out ChIP's (chromatin IP) without fragmenting the chromosomes but it 
doesn't seem to work. Even the negative control (beads only) ppts the 
DNA!! I would really appreciate if anyone has any advice or 
suggestions.

Thanks,
Shwetal
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SHWETAL MEHTA
Graduate student
Department of Microbiology
University of Texas at Austin
Austin, Texas - 78712

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