On 2 Aug 2001, vuk wrote:
> When plated on selective media (SD-agar, -Trp), my Y190 yeast strain
> transformed with PGBT10-clusterin construct grows in only one night.
> The same happens when I sequentially cotransform this construct with a
> cDNA bank.
> Has anyone any idea why is it so and should I consider this an
> irregularity or not?
I would check the phenoptype (-ura, -leu, or whatever) and have a look at
them under the microscope. My bet is that whatever you have is not what
you think it is. Seeing colonies from a transformation after one night is
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