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Premature yeast growth

Alex Brands abbrands at artsci.wustl.edu
Thu Aug 2 12:29:58 EST 2001


On 2 Aug 2001, vuk wrote:

> When plated on selective media (SD-agar, -Trp), my Y190 yeast strain
> transformed with PGBT10-clusterin  construct grows in only one night.
> The same happens when I sequentially cotransform this construct with a
> cDNA bank.
> Has anyone any idea why is it so and should I consider this an
> irregularity or not?
> Thanks
> Vuk

I would check the phenoptype (-ura, -leu, or whatever) and have a look at
them under the microscope.  My bet is that whatever you have is not what
you think it is.  Seeing colonies from a transformation after one night is
very unlikely.



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