Thanks for the suggestions. My blank = yeast cells with lacZ but no
estrogen. I incubate for 15min and get yellow color development within an
hour for both the blank and spiked samples. My "no yeast cells" control has
no cells, no added estrogen and no color development even after days so I'm
thinking it's probably not an ONPG problem.
Eleanor.
==
>From: David Jansma <davidjansma at hotmail.com>
>To: yeast at net.bio.net>Subject: Re: liquid beta-gal assay
>Date: 26 Apr 2001 00:51:07 +0100
>>Hi Eleanor,
>Does blank = yeast cells without the lacZ gene? If it is a control without
>cells, how long do you incubate after adding ONPG? Old ONPG solutions can
>hydrolyze spontaneously, so it is a good idea to make them fresh. If you
>are incubating for long times, try increasing the concentration of your
>cells instead; that may reduce the colour that you are seeing in your
>control.
>David
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