Hi,
I am growing S. cerevisiae in 400ul microtiter wells (96-well
format) with the goal of doing growth curves. (I have access to a plate
reader.) I place the plate on an orbital shaker, set to about 375rpm (this
is sufficient to allow the culture to swirl up the sides, hopefully
preventing aerobic growth). The problem is that the yeast accumulate on
the bottoms of the wells (and sometimes along the edge of the
bottom). This interferes with the ability to get accurate readings of
absorbance. I'm sure that at the speed I'm shaking them, they shouldn't be
settling out... my only guess is that I'm setting up a vortex, resulting in
the accumulation. (Not very convincing, though.)
Has anyone else tried to do this? Any suggestions as to how to
avoid this accumulation?
Thanks in advance for any help.
-Rachel
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Rachel N. Fish, Grad Student
MCB, UC Berkeley
Lab: (510)643-6136
rfish at uclink4.berkeley.edu
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