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FOA dosage

jthompson at georgian.edu jthompson at georgian.edu
Fri Sep 29 16:30:14 EST 2000

Does anyone have any experience with increasing 5-FOA dosage for use with S.
cerevisiae?  I have a new batch of 5-FOA that seems to be allowing leaky growth
of ura+ cells.  It's fairly obvious that they grow much slower than ura- cells
on FOA media (and never really make it up to full size colonies), but the
background is causing some problems with my screening.  Has anyone tried FOA at
doses higher than the standard 1 mg/ml?

Jeff Thompson

Dr. Jeffrey S. Thompson
Assistant Professor of Biology
Georgian Court College
Lakewood, New Jersey USA


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