I am working with the yeast one hybrid (the kit is from Clontech). I am
trying to construct lacZ reporter yeast strain. I have to screen for
transformants using the beta Galactosidase assay via the colony lift method.
I don't seems to have any positives from test. The positive control from the
kit also does not show any color change. I have tried the protocol from
Current Protocol and also protocol from Clontech. Could it be the low
expression? I intend to use the strain for my screening.
Universiti Putra Malaysia
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