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5' end mapping

David J. Meyer, Ph.D. meyerdj at phibred.com
Mon Sep 25 15:42:11 EST 2000

These references describe the procedures in detail

Boorstein, W., and Craig, E. (1989). Primer extension analysis of RNA. Meth
Enzymol 180, 347-369.

Calzone, F., Britten, R., and Davidson, E. (1987). Mapping of gene
transcripts by nuclease protection assays and cDNA primer extension. Meth
Enzymol 152, 611-632.

I believe that some companies (try Ambion) are now making kits to do this
for the biochemically-challenged ;-)

David J. Meyer, Ph.D.
Pioneer Hi-Bred International, Inc.

C Hurtado wrote in message ...
>Hi folks,
> Does anybody have a reliable protocol to map the 5'-end of a low
>abundant yeast mRNA? I've been trying the standard 5' anchored PCR
>protocol, without success. Any help will be highly appreciated.
> Thanks in advance,
> Cleofe
>Cleofe Antonio Rodriguez Hurtado, M.Sc., Pharm.D.
>University of Alberta
>Department of Cell Biology
>5-14 Medical Sciences Building
>Edmonton, Alberta
>Canada T6G 2H7
>TEL: (780) 492-7407
>FAX: (780) 492-0450

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