IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

32P labeling of S. Cerevisiae

Michael J Conboy conboymj at Stanford.EDU
Wed Sep 13 14:09:30 EST 2000

Yeast can have substantial reserves of phosphate (polyphosphate), in the
vacuole, and will not label efficiently until the reserves are used up.  
How long are you growing them in -P media, and it it P-limited or truly
-P?  Before you break out the P-32 again, you might want to passage a
culture for some time in -P medium, until it slows in growth, just to see
how long it takes to become phosphate depleted.  Or, if you have a pilot
culture growing a number of days ahead of your experimental culture, both
in -P medium, then you'll know ahead of time when to switch to P-limited

Hope this helps,

In article <8pn31g$dg0$1 at mercury.hgmp.mrc.ac.uk>,
Brian Baker  <brb at chem.ucla.edu> wrote:
>I'm having a hard time trying to label my yeast cells with 32P. the
>label does not seem to be incorporated. I'm using 32P in the form of
>H3PO4, I'm growing my cells in -phosphate media, and I label them for
>~45 min at 30 C. Does anyone know what's I can do? or have a better

YEAST bionet newsgroup see: http://www.bio.net/hypermail/YEAST/
YEAST e-mail: messages sent to yeast at net.bio.net
subscribe: e-mail biosci-server at net.bio.net with: subscribe yeast
unsubscribe: e-mail biosci-server at net.bio.net with: unsubscribe yeast
YEAST on the WWW: http://genome-www.stanford.edu/Saccharomyces/VL-yeast.html
problems with the YEAST newsgroup? E-mail the moderator: francis at cmmt.ubc.ca

More information about the Yeast mailing list

Send comments to us at biosci-help [At] net.bio.net