IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

RT-PCR

K.Fushimi fushimi at ncnp.go.jp
Tue Nov 21 02:47:06 EST 2000


I plan to do RT-PCR  with S.cerevisiae mRNA.
But I have no experience other than mammalian mRNA.
Dose it work protocols for mammalian?
For example the concentration of oligodT or random primers.
Which is better, oligodT or random random primers?
What's different between yeast and mammalian cells

Please tell me your experience!
Thank you.

postdoc
Kazuo Fushimi
National Institute of Neuroscience, NCNP, Japan(NIN)
fushimi at ncnp.go.jp



---




______________O_________oO_____________oO______o_______oO___________________
YEAST bionet newsgroup see: http://www.bio.net/hypermail/YEAST/
YEAST e-mail: messages sent to yeast at net.bio.net
subscribe: e-mail biosci-server at net.bio.net with: subscribe yeast
unsubscribe: e-mail biosci-server at net.bio.net with: unsubscribe yeast
YEAST on the WWW: http://genome-www.stanford.edu/Saccharomyces/VL-yeast.html
problems with the YEAST newsgroup? E-mail the moderator: francis at cmmt.ubc.ca







More information about the Yeast mailing list

Send comments to us at biosci-help [At] net.bio.net