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K.Fushimi fushimi at ncnp.go.jp
Tue Nov 21 02:47:06 EST 2000

I plan to do RT-PCR  with S.cerevisiae mRNA.
But I have no experience other than mammalian mRNA.
Dose it work protocols for mammalian?
For example the concentration of oligodT or random primers.
Which is better, oligodT or random random primers?
What's different between yeast and mammalian cells

Please tell me your experience!
Thank you.

Kazuo Fushimi
National Institute of Neuroscience, NCNP, Japan(NIN)
fushimi at ncnp.go.jp


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