Hello all,
I am trying to do invertase assays in lab, but I'm having a problem with
the sucrose. The problem is that the sucrose is contaminated with
glucose. Since the assay is suposed to measure how much glucose is
liberated from sucrose, this is a big problem as it gives me a very high
background signal. I tried sucrose from other labs, and they all have the
same problem.
Can anyone tell me what is the best way to make a glucose free sucrose
solution? My first option right now is to treat the sucrose solution with
glucose oxidase and catalase, but I'm hoping there is a simpler way.
Thanks!
Alex Brands
Washington University
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