Hi
I'm trying three hybrid system with pBridge plasmid(Clontech).
The plasmid have Met 25 promoter which is activated in the absence of
methionine.
I constructed some plasmids with pBridge and transformed into AH109
strain.
But the cells could not grow in the absence of methionine.
I used SD medium (-Trp, -Met).
I'm not specialist for yeast research so I need more information.
Can S. cerevisiae grow in the absence of methionine?
Do I need to add anything as substitute for methionine?
If you have some experience using pBridge,
Please tell me your case.
Kazuo Fushimi
National Institute of Neuroscience, NCNP, Japan(NIN)
fushimi at ncnp.go.jp
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