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cell disruption via ultrasonics

Suzanne Elsasser elsasser at flash.net
Thu May 18 22:16:43 EST 2000


> Has anyone experience with ultrasonic devices for cell disruption of
> plants/fungis (for DNA extraction)?
>
> Any suggestion? Tips, Tricks, Hints????????????

If you are using standard laboratory strains, sonication pretty much serves to
separate clumps of cells, but does not lyse them.  Spheroplasting followed by
sonication should allow for lysis.  Depending on what you want to do with the
DNA, purification may not be necessary.

Suzanne


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