Alex Brands <abbrands at artsci.wustl.edu> wrote:
> I have been doing some 2-hybrid experiments, and I would like to do
> Western Blot analyses to determine if the fusion proteins are being made.
> To do this I need antibodies to the Gal4BD and Gal4AD. Clontech sells
> these, but they charge $225 for an amount sufficient to do 10 blots. This
> seems very expensive compared to other antibodies I have priced.
In addition to the rather high price the BD antibody is less than optimal
(politicaly correct phrase used) - avoid it if you can.
Which vectors do you use for your experiments? At least the fish vector
should contain a tag (e.g. HA).
Dr. Philipp Pagel
Cellular and Molecular Physiology phone: (203) 785-6835
333 Cedar ST
New Haven, CT 06520
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