On a similar note. Does anybody have a Saccharomyces strain with GFP
integrated into the genome? Are you willing to lend it out?
Cheers,
Alex Grant
On 31 Mar 2000, B.Drees wrote:
>>> I am thinking about tagging some yeast proteins with GFP or GFP variants
> for localization. I'm trying to decide on the best strategy to use for
> this. My main questions are:
>> - Integrate tag into genome or express fusion on plasmid vector? So far
> my integrated tags are invisible so I am leaning toward using plasmids.
>> - N-terminal or C-terminal fusions? Will an N-terminal fusion screw up
> localization signals?
>> - Inducible or constitutive promoter on the vector?
>> Thanks!
>> Becky Drees
>
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