I am thinking about tagging some yeast proteins with GFP or GFP variants
for localization. I'm trying to decide on the best strategy to use for
this. My main questions are:
- Integrate tag into genome or express fusion on plasmid vector? So far
my integrated tags are invisible so I am leaning toward using plasmids.
- N-terminal or C-terminal fusions? Will an N-terminal fusion screw up
localization signals?
- Inducible or constitutive promoter on the vector?
Thanks!
Becky Drees
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