In article <8b1goj$4b3$1 at mercury.hgmp.mrc.ac.uk>,
Ashok Aiyar <a-aiyar at nwu.edu> wrote:
>> We extract plasmid DNA from yeast by vortexing in the presence
> of acid-washed glass beads, Triton X-100, SDS, and phenol-chloroform.
>> This method has never worked particularly well for me, and we are
> close to running out a batch of acid-washed beads that was gifted to
> me several years ago.
>> So I have two questions:
> a) what grade glass-beads should I buy, and how do I acid-wash them?
> b) Are there any alternative methods that work more efficiently and
> reproducibly?
I use the same method, and also had trouble until I started
precipitating the supernatant with ethanol/sodium acetate after
vortexing/centrigugation. I use pre-washed beads purchased from Sigma.
Works great!
Ed Davis, Ph.D.
National Cancer Institute
NCI-FCRDC
P.O. Box B
Frederick, MD 21702-1201
Tel (301) 846-1564
FAX (301) 846-6911
email: davise at ncifcrf.gov
Sent via Deja.com http://www.deja.com/
Before you buy.
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