Koi Yau Lam schrieb:
>> I am screening a cDNA Library for interactions and I plan to PCR sequence the insert to identify the protein
> Does anybody has any experience with this? I need to know if the plasmid DNA obtained is pure enough or in sufficent quantities to directly sequence it with out amplifying with PCR
> Thanks
> Kevin
> ---
Do you mean the plasmid DNA recovered from the positiv yeast colonies?
If so, this has
usually to be transformed into E. coli first since quantities are pretty
low not even
enough to transform subcloning grade competent E. colis.
Ricky
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Frederik Boernke
Research Group of Molecular Plant Physiology
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Fax. 039482 -5 515
http://www.ipk-gatersleben.de
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