We extract plasmid DNA from yeast by vortexing in the presence
of acid-washed glass beads, Triton X-100, SDS, and phenol-chloroform.
This method has never worked particularly well for me, and we are
close to running out a batch of acid-washed beads that was gifted to
me several years ago.
So I have two questions:
a) what grade glass-beads should I buy, and how do I acid-wash them?
b) Are there any alternative methods that work more efficiently and
reproducibly?
Many thanks!
Ashok
--
Ashok Aiyar
Assistant Professor Email: a-aiyar at nwu.edu
Department of Microbiology-Immunology Tel: (312) 503 2524
Northwestern University, Chicago, IL 60611 Fax: (312) 503 1339
______________O_________oO_____________oO______o_______oO___________________
YEAST bionet newsgroup see: http://www.bio.net/hypermail/YEAST/
YEAST e-mail: messages sent to yeast at net.bio.net
subscribe: e-mail biosci-server at net.bio.net with: subscribe yeast
unsubscribe: e-mail biosci-server at net.bio.net with: unsubscribe yeast
YEAST on the WWW: http://genome-www.stanford.edu/Saccharomyces/VL-yeast.html
problems with the YEAST newsgroup? E-mail the moderator: francis at cmmt.ubc.ca
