I have a construct with over 100bp of 5' DNA and it expresses just fine on a
Gal -inducible vector. I heard though that you should check if your 5'
sequence has a lot of secondary structure (eg loops)
Younes
James Harper <jah49 at cam.ac.uk> wrote in message
news:8alhfu$85l$1 at mercury.hgmp.mrc.ac.uk...
> Dear Yeast People,
>> I'm using two different galactose inducible vectors (pYES2 and pKV49).
> I was wondering if anybody knows if the length of the untranslated
> region between the Gal promoter and ATG is related to the level of
> protein expression.
>> Any information gladly received.
>> James
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