David Humair schrieb:
>> Hi netters,
> Does somebody used two-hybrid system (THS) to screen for proteins that
> interact (receptor-targeting peptide) in the lumen of Golgi or vesicles
> or vacuole/lysosome?
> My question is: Could conditions in these compartments (pH, etc) be
> responsible of the fact that no positive clones are detected! I mean, in
> nucleus, where THS occurs, pH is certainely different!
>> Thanks a lot
>Couldn't it be that proteins in the secretory pathway are glycosylated
how which doesn't occur in the conventional THS?
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