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Two-hybrid system for proteins localised in the vacuole

Frederik [iso-8859-1] Börnke ricky_boernke at gmx.net
Thu Jan 20 15:16:09 EST 2000



David Humair schrieb:
> 
> Hi netters,
> Does somebody used two-hybrid system (THS) to screen for proteins that
> interact (receptor-targeting peptide) in the lumen of Golgi or vesicles
> or vacuole/lysosome?
> My question is: Could conditions in these compartments (pH, etc) be
> responsible of the fact that no positive clones are detected! I mean, in
> nucleus, where THS occurs, pH is certainely different!
> 
> Thanks a lot
> 
> David
> 
Couldn't it be that proteins in the secretory pathway are glycosylated
some
how which doesn't occur in the conventional THS?

Ricky





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